FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_LPHK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_LPHK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2602302
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC1950.059.2273565
GCGATAA8700.058.329975
CGGATAG8750.057.1967055
CGCTACG2450.057.1395656
GATAGGT20750.056.8390057
CGGGATA8200.056.3382154
CCCGTCC6650.055.78411510
GGATAGG18150.055.7268146
TCCCGTC5850.055.0374539
GCGATAG10650.054.550855
GATAGGG19400.054.480357
TGATAGG24800.054.0493056
GGGCTAC3700.053.9158135
GGGATAG16750.053.907345
CGCGATA5400.053.7931944
CAGATAG12300.053.492855
GCGGTAC2000.052.4969755
GGATAGT20350.052.453986
GATAGTC11500.052.3438037
GATAGGA24600.052.0691687
Produced by FastQC (version 0.11.5)