FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPLK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPLK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2790019
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA6000.060.080074
CTGATAG15200.058.4836655
GCGATAA9000.058.330175
CCTCGAC2200.057.269625
ACGATAG9050.057.234465
CGGGATA7650.056.271464
CGGATAG9100.056.15085
CAGATAG13800.056.0476885
CCGATAA7750.055.9969565
GCTGATA16250.055.566224
GCGATAG9750.054.9200945
CCTGATA8800.054.8833854
CGATAAT13100.054.768026
CGATAGG9350.054.275136
CGATAGT10600.054.1480036
GATAGGT19950.054.031197
GATAGGA22100.053.6839077
CGATAGC4900.053.568526
CCTATAG6350.053.4616625
GATAGTC9900.053.3799867
Produced by FastQC (version 0.11.5)