FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPLK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPLK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2782185
Sequences flagged as poor quality0
Sequence length76
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGGA44900.061.3414847
TGATAGG37350.060.0611046
CAGATAG13950.059.7074665
TCCCGTC16200.059.6238449
GCGGTAC2300.059.342075
GTAGGAC11250.059.1053738
GGATAGG19450.059.017416
GATAGTC12600.058.8831757
CCGCTAC3750.058.7942925
GCTGATA22450.058.7693524
CGCTACC3600.058.327676
CGCTACA4200.058.327676
GCGATAG11500.058.1247945
CCCGTCC19300.057.662810
GCTCCCG7850.057.510347
CTATAGG13700.057.2207266
CGGGATA9650.056.937484
CGCGATA6800.056.6121524
GGGATAG17900.056.307395
CGGATAG10950.056.2502485
Produced by FastQC (version 0.11.5)