FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPLK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPLK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594182
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA4900.059.9965484
GCGATAG9400.057.7094425
CGGATAG8450.057.1564755
CGATAGT10850.057.0934876
CCCGTCC5500.056.63310210
CTGATAG15500.056.4483645
CCTGATA7150.056.2904664
GATAGGA23050.056.1789787
GCCGATA4500.055.996784
GCTGATA15750.055.330154
GGATAGG15100.055.162396
GATAGTC8900.055.0530177
GGATAGT18500.055.0508886
TGATAGG21300.054.7151646
CGGGATA7100.054.2222334
GATAGTG19300.054.2196777
ACGATAG8650.054.216535
CGATAGG10300.053.686236
TATAGCG4700.053.6139347
GATAGGT18450.053.492867
Produced by FastQC (version 0.11.5)