FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPLK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPLK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2413586
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATAG10650.056.8517045
CGGATAG9000.056.386155
CGCGATA4350.055.5145454
GGGGTAC3350.055.3704455
ACGATAG5950.055.291435
GATAGGT15200.055.261627
GGATAGG17300.055.229686
GGATAGT17700.054.7724886
CCGCTAC2700.054.4418035
CCCGTCC6000.054.24736810
GGGATAG16300.054.1078075
GATAGTC10200.053.527927
TGATAGG18000.053.2762956
GCGATAG8050.053.0409055
CGATAGT8800.052.8962556
GGATAGC5450.052.6590846
GATAGGG16550.052.445677
GATAGGA20250.051.5047387
TCCCGTC5600.051.246459
CCCGATA2950.051.0144774
Produced by FastQC (version 0.11.5)