FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPLK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPLK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2729483
Sequences flagged as poor quality0
Sequence length76
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG9100.059.2292335
CGTATAC3300.058.3318255
CGCGATA7150.058.2502374
CGGGATA8150.057.5445254
CGATAGT10000.057.3985146
GGATAGT18700.055.7739566
GATAGGT20000.055.2965437
CCCGATA5100.054.900544
CAGATAG12500.054.8785745
GGATAGG18950.054.8534626
TGATAGG19650.054.5024036
CCGATAA8300.054.3961835
GCGATAG9700.054.122315
TATACGG4400.054.087537
GGGTCCC2150.053.719546
CGATAGG9450.053.7023126
CCGTATA5550.053.6022154
CTGATAG15250.053.244525
CCGATAC1650.053.028935
GATAGTC10550.052.7455187
Produced by FastQC (version 0.11.5)