FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPLK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPLK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981303
Sequences flagged as poor quality0
Sequence length76
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTCC1700.061.7631235
TCCCGTC10750.060.5525259
GATAGGA41550.059.550237
CAGATAG15500.059.385575
TGATAGG35550.058.0857126
CCCGTCC12850.057.1931410
GCGATAA10300.057.0859155
CTAGGAC10800.057.031758
GGATAGG20950.056.466336
CGACCGT3350.056.412688
CGGGATA9250.055.9985664
CGGCTAC3750.055.998565
CGGATAG11350.055.5051845
GATAGTC11750.055.4000437
CCGCTAC2850.055.2617385
CGCTCCC3050.055.0805556
GATAGGT19450.055.060097
GTAGGAC10650.054.877548
CCTGATA10700.054.6247674
ACGATAG11150.054.6174355
Produced by FastQC (version 0.11.5)