FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPK-3S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPK-3S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3579947
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG12250.059.9971055
GATAGGA30450.058.2722367
GGATAGT23300.057.3792086
CGATAGG12750.057.3697786
CGGGATA12150.056.746234
GCTACCG4600.056.3008427
GGATAGG25500.056.1345446
CGCGATA7200.055.9000784
CTGATAG18950.055.7756735
CGATAGT13400.055.1092836
CCGATAC2800.054.9973455
GATAGTC13300.054.9965747
TGATAGG27850.054.5395556
GATAGGG21700.054.5127337
GATAGTG24000.054.392457
CAGATAG18950.054.1134835
GGGATAG23900.053.888615
GTGATAG24450.053.5352485
TGGATAG25300.053.5349665
CCGATAA11000.053.4519655
Produced by FastQC (version 0.11.5)