FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPK-3R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPK-3R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2659977
Sequences flagged as poor quality0
Sequence length76
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCCCC1800.058.328387
GATAGTC10650.056.5210277
CGGATAG9250.056.3767745
GCGATAA9250.056.3767745
CTGATAG16800.055.8317455
CGGGATA8550.055.670934
CAGATAG13400.055.3715555
GGATAGT18900.055.3687936
GGATAGG17150.055.304556
ACGATAG8250.055.1499445
GATAGGT19450.055.0595937
TGATAGG26450.054.2517666
GGTATAG11750.053.9133575
CGATAGT11050.053.844626
GATAGGG18200.053.8415837
CGCGATA7050.053.6154984
GGGATAG17750.053.4350975
GATAGGA25250.053.0846027
GTGATAG24050.051.9527855
CACGATA6100.051.6378754
Produced by FastQC (version 0.11.5)