FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPK-2S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPK-2S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3226191
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG12250.059.9988865
CAGATAG17050.057.4769365
CGGGATA10800.057.3600464
GGATAGT23100.057.271666
ACGATAG9450.057.035985
GATAGTC12100.056.4030347
CGATAGG12600.056.110076
GGGATAG20700.055.965145
CCGATAA10100.055.7910425
GGGGATA19250.054.1808134
CGATAGT10550.054.074826
GATAGGG19450.053.9827357
GGATAGG23750.053.788476
CCTGATA9450.053.3323444
GCCGATA6900.053.259884
CGCGATA5850.053.2468724
CCCGATA5850.053.2468724
GATAGGT24650.053.2436187
GTGATAG23350.053.061115
TGATAGG22250.052.8529476