FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPK-2R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPK-2R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2453688
Sequences flagged as poor quality0
Sequence length76
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTAC2800.062.4955375
GGATAGG14750.060.5041586
CCCGTCC11700.060.12269210
GATAGGA26000.057.4766737
CTAGGAC6900.056.8063858
TGATAGG24000.056.7251246
GCGATAA9350.056.520035
TCCCGTC11200.056.244849
CGATAAC4000.055.996016
CGCTACC2750.055.9960026
CAGATAG12500.055.7160265
CGCGATA5100.055.5831384
CGATAGT9850.055.4275176
CGGATAG9600.055.4127165
GATAGGT14500.055.0305567
GATAGTC12500.054.8760837
GATAGGG17050.054.8054737
GGTCCCC1600.054.68367
GGATAGT17150.054.4859126
CCTATAG5650.053.889965
Produced by FastQC (version 0.11.5)