FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPK-1S.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPK-1S.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3460759
Sequences flagged as poor quality0
Sequence length76
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA11900.058.2338754
CGGATAG11450.057.7715155
CGCGATA8250.057.2713284
CGATAGG11200.057.186116
ACGATAG10500.056.331965
GGATAGT24800.056.026866
GATAGTC12650.055.6088837
GCCGATA7750.055.547044
CGATAGT14900.054.495326
GGGATAG22600.054.3570825
CAGATAG16550.054.349135
GATAGGT26000.053.4386867
GCGGATA14550.053.1602174
CTGATAG17850.053.1359635
GGTATAG15200.052.728985
GTATAGG15700.052.6101846
GATAGGG22050.052.5361257
GATAGGA26400.052.4964457
GGATAGG23300.052.423626
GCGATAG13450.052.3035625
Produced by FastQC (version 0.11.5)