FastQCFastQC Report
Thu 3 Dec 2020
HV2Y7BGXG_n01_col4-0.5_HPK-1R.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2Y7BGXG_n01_col4-0.5_HPK-1R.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3720930
Sequences flagged as poor quality0
Sequence length76
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATAGG20900.059.6138046
GATAGGG18050.059.1378827
CGGGATA9750.058.8676264
CGCGATA7500.058.32924
GGATAGT29000.058.048396
CTGATAG17050.057.0634045
GGCGATA11800.056.9451144
CAGATAG13400.056.9362645
GCGATAA13050.056.854215
GGGATAG21150.056.7572065
CGGATAG11000.055.3597155
CACGATA8600.055.3449174
GATAGGT27100.055.1444367
GATAGTC13600.053.5263377
GCGGTAC2550.053.525625
CCTGATA11150.053.045574
TGGGATA28050.052.5274734
CGCTACA5150.052.3270956
CGATACG2350.052.124676
GATAGCG6450.052.0900347
Produced by FastQC (version 0.11.5)