FastQCFastQC Report
Tue 30 Jul 2019
HV2TMBGXB_n01_8_EV_R2_RUN2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2TMBGXB_n01_8_EV_R2_RUN2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15543798
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT4645352.9885553067532147No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT3798512.443746373955709No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA3101861.995561187812657No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA2973331.912872259405327No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC2964111.9069406331708634No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG2423241.5589754833406866No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG2331851.5001803291576485No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG2252221.4489508934688935No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA2106241.3550356225679205No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA2006161.2906498141573894No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC1684281.0835704375468596No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG1597911.0280048672788982No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA1517380.9761964225217029No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG1495490.9621136352904226No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT1441610.9274502923931461No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA1349140.8679603273279799No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG1295900.8337087242127053No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA1287920.8285748438058703No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC1269710.8168595603211004No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC1227540.7897297687476381No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA1151400.7407456015576116No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA1058780.6811591349810387No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1040290.669263715341643No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA1027380.6609581519265755No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA1025070.6594720286509127No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT925540.5954400591155392No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT846000.544268524333628No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA814760.5241704762246653No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT790440.508524364508597No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT785100.5050889107025195No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT764480.4918231696011489No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG754500.48540260237555843No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA712660.45848511412719084No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC681710.43857363560694756No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT664170.4272893922064608No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT648570.41725323502016687No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC629230.4048109734828No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA582390.37467676818754336No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT560750.36075481680860755No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT551910.35506766106970766No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA534640.3439571203897529No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT532120.3423358949981208No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC514980.3313089889613851No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC487570.3136749461103393No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG477570.3072415120165612No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA475600.30597412550008696No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT450930.2901028435907363No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG443600.28538713639999697No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT437040.2811668036344785No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT428680.27578845273208No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT389490.2505758245185636No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC376050.2419292890965258No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT370840.23857746993366744No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG370190.23815929671757186No Hit
TGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTA367500.23642870294634555No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG363920.23412553354077298No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC357710.2301303709685368No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA355260.22855417961556113No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG353650.22751839672646287No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT339830.2186273908088615No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC333130.21431698996603019No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG327010.21037972830063797No Hit
TGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA325670.20951764813207172No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG318560.2049434764913955No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC312850.20126998562384818No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA305340.19643847661942082No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT304740.1960524705737941No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG300880.19356916501359578No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA298130.1917999706378068No Hit
AACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC291050.18724509929941188No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT286790.18450445637546242No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGG285990.18398978164796018No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT285730.18382251236152194No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG275950.17753061381780694No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA271830.17488003897117035No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA268670.17284707379753647No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC267510.17210079544265822No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA264940.17044740288055724No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA259190.16674817827663485No Hit
TACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACAT247930.15950413148704068No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG247850.15945266401429045No Hit
CACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAG234770.15103773221962868No Hit
CCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTA224520.14444346227350613No Hit
TACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTC214060.13771409021141423No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCT213360.13726374982484973No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA210480.13541092080584166No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG208370.13405346621205447No Hit
TACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGA203570.130965417847041No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG202320.13016123858531872No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG197810.1272597598090248No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG195720.12591517208342518No Hit
TAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC193250.12432611386226197No Hit
GCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGCGGA189910.12217734687494008No Hit
TATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACT188970.12157260407012495No Hit
ACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACA184650.1187933605416128No Hit
CCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGT177920.11446365939650013No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA177320.11407765335087344No Hit
AGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAGGC170780.10987018745354257No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATC169730.10919467687369586No Hit
CATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACAC166220.10693654150677975No Hit
GAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAG161270.10375199163035959No Hit
TGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAA157570.10137162101566168No Hit
CTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT157380.1012493857678799No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG156910.10094701436547232No Hit
ACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCG156640.10077331164494031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAC275450.062.578931
TAGGGAA154700.061.369451
TTGTACT866650.060.0850071
TGTACTC899000.058.264672
ACTCTAG967250.054.1677675
GTACTCT984700.053.197193
AGGGAAG180950.053.0937042
TACTCTA995400.052.7589654
TGTTACT333200.051.9107132
CTCTAGT1018050.051.3651356
GAATAAT116450.051.1950571
TTTTTCC324950.049.6742631
TCTAGTT1067350.048.769647
TAATACG124550.047.124554
GTTACTA392850.044.0641333
ATAATAC137500.043.068083
AATACGA139450.042.2901575
CTAGTTG1235800.041.9319088
TAGTTGT1267450.040.964899
TTACTAC437250.039.5896954
Produced by FastQC (version 0.11.5)