FastQCFastQC Report
Tue 30 Jul 2019
HV2TMBGXB_n01_6_ABF3_R3_RUN2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2TMBGXB_n01_6_ABF3_R3_RUN2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16991589
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT3191621.8783528721180813No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT2829991.665524042513034No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC2059291.2119466872698015No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA2037081.198875514232365No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA1855101.0917754660850143No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG1591540.936663428005468No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG1588100.9346388969271797No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA1520080.894607326012888No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG1345320.7917564390240371No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA1314810.7738004962337542No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC1138360.6699549995000468No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG1071080.6303589381781775No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT1010060.5944470525976118No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA1002540.5900213334962374No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG962840.5666568323892486No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA895320.5269195247130801No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA889410.5234413332384629No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC784140.4614871510839863No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC778840.4583679607598795No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG774240.4556607389691453No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA731950.43077195428867776No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG727670.42825306097034244No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA723840.42599900456631806No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA688170.40500626515860294No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA668440.393394637782258No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT648750.38180655146496306No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT616060.36256762095646267No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT529220.31145998175921036No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA505540.29752367480169156No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT481970.2836521057565599No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT470160.2767016080720879No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT452020.2660257377929751No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA446730.26291243273363074No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT440880.2594695528475883No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG437880.2577039734188486No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC410790.24176079117732896No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT400020.2354223610281534No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC394910.2324149907345334No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA386890.2276950083950359No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT359330.21147521870968045No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC346040.20365370184036347No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT332010.1953966753786241No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA323650.19047659403720274No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC316560.18630394132061456No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT305480.17978306796380256No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT305310.17968301846284065No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG292820.17233232277452099No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC287830.16939557565805058No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA285030.16774770152456017No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG277590.1633690645412857No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT266460.15681876486066135No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT258020.1518516014011403No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG257940.15180451928304056No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG239760.1411051079448779No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC239230.14079318891246723No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT238620.14043418776195682No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT233990.1377093101769352No Hit
TGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTA231410.13619091186821902No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG228410.13442533243947932No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC225000.1324184571554785No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG224780.13228898133070427No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC216070.12716291572259664No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG210020.12360233054130487No Hit
TGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA209180.12310796830125777No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG203750.11991226953523887No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA200840.11819965748936134No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGG198190.11664006232730793No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA193680.11398580791943591No Hit
TTTTAATCATAAAAACCCATCTCATAAATAACGTCATGCATTACATGTTA192580.11333842879556469No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA188950.11120207768678962No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA188490.1109313555077162No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA184880.10880677492846609No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT183130.10777685359503457No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT182330.10730603241403733No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA173410.1020563762459179No Hit
TACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACAT171510.10093817594104942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAC209900.057.4719921
TTGTACT641950.056.806091
TGTACTC648200.056.4946482
ACTCTAG701700.052.5763555
TACTCTA698450.052.5002944
GTACTCT700250.052.315363
TAGGGAA112850.052.176851
CTCTAGT731150.050.190566
TCTAGTT780300.047.0102237
TGTTACT285850.042.2783972
CTAGTTG878350.041.4037488
TAATACG84650.040.3536644
TAGTTGT919450.039.7661369
AGGGAAG150950.039.7177242
GTTACTA312250.038.7038543
AGTTGTT970600.037.45784810
TTTCCAC661900.037.344551
TTTTTCC287500.035.9566351
TTACTAC349850.034.7942774
ACTACCC348250.034.652636
Produced by FastQC (version 0.11.5)