FastQCFastQC Report
Tue 30 Jul 2019
HV2TMBGXB_n01_18_EV_R3_RUN1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2TMBGXB_n01_18_EV_R3_RUN1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18664968
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT4002942.144627303941802No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG3770812.020260629431564No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA3657471.9595372464608567No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT3652821.9570459483241547No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA2862571.5336592058448748No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG2771571.4849047691911392No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC2717961.4561825126086474No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA2509071.3442669711515176No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA2201901.1796966381083536No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA2020831.082686024428223No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA1636070.8765458370997474No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC1515640.8120238941743698No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG1495270.8011104010464951No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG1406260.7534221328426601No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT1367950.7328970507744776No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG1367870.7328541897312656No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA1270070.6804565644045036No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG1206670.6464891876589342No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1170370.627040989301455No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC1114160.5969257488145707No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA1043840.5592508918311566No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA962810.515838012687726No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA911610.4884069450319979No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT889300.47645407160623043No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT871620.46698178105636184No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT865830.4638797130538879No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA797980.4275281907796467No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG784460.42028467447680595No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT782900.41944888413417053No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT721850.38674055053295564No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC696720.37327682533396256No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA689360.3693336093584516No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA682630.3657279240982358No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA676370.3623740474668909No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC640720.34327409508550993No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT627620.33625559925953263No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC593780.3181253779808248No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT585050.3134481666403071No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG552290.29589656944496234No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT545210.2921033671206937No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC543190.29102112577958883No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT527740.2827435868092568No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC519000.27806101783833753No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC508840.27261766535040405No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT458190.24548126736675893No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG455480.24402934952794988No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG447950.23999505383561331No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG442470.23705907237558618No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT428920.22979948318154092No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG411840.22064865045576287No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT401150.21492134355654935No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC381260.20426501668794717No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT361290.1935658287761329No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA352050.18861537828513822No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG350620.1878492371377224No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG349080.18702416205588993No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC327760.17560169403987191No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA323720.17343721135766213No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC320550.17173884252038363No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG318830.17081733009132402No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT306630.16428102100148256No Hit
TGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTA301590.16158077527912185No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT298920.16015028796191882No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGG296350.1587733769487309No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG294670.1578732950412773No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG294370.15771256612923204No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG294300.15767506271642148No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA294260.15765363219481546No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT291100.15596062098793848No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG280200.15012080385029325No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT279870.14994400204704342No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG279370.14967612052696797No Hit
AACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC275030.14735090893271288No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG269490.14438278169027668No Hit
CCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTA263600.14122713738378764No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA263180.14100211690692424No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT252850.13546768470216505No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA250400.13415506525379525No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT246750.1321995301572443No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA244960.13124051431537412No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA243300.13035114766872358No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG226650.12143069305021043No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC222770.1193519324544248No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT213150.11419789200817275No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA210350.1126977554957501No Hit
TGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA207010.11090830694164598No Hit
ACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACA206070.1104046896839041No Hit
GAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAG205470.11008323185981353No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA203730.10915100416995091No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA202040.10824556463209581No Hit
TACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTC201550.10798304074242185No Hit
AGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATA196460.10525600686805356No Hit
CTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT192090.10291472238259396No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCT189000.10125921458852755No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA188490.10098597543805059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAC456000.062.983681
TAGGGAA148950.057.2302551
TTGTACT961150.057.1695941
TGTACTC995000.055.4546622
TGTTACT545400.052.846432
ACTCTAG1056650.052.0803345
GTACTCT1090650.050.594363
TACTCTA1104000.049.954434
AGGGAAG175750.049.2090072
CTCTAGT1119200.049.010176
TCTAGTT1182150.046.3069657
TTTTTCC425750.046.1784861
GTTACTA625250.046.1477243
GAATAAT139300.044.5327841
TAATACG137050.043.9257244
TTACTAC682700.042.244174
ACTACCC696900.041.207636
TACTACC702200.041.02625
ATAATAC154150.039.188923
CTAGTTG1442450.037.838748
Produced by FastQC (version 0.11.5)