FastQCFastQC Report
Tue 30 Jul 2019
HV2TMBGXB_n01_13_ABF3_R1_RUN1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2TMBGXB_n01_13_ABF3_R1_RUN1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18886814
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT6310203.341061123384812No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT6250843.309631788611885No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG4665172.4700672119712728No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC4517792.392033934362884No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG4198862.2231700910487073No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA3969552.1017573424506644No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA3752321.986740590551694No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA3713841.966366587821535No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA3139711.6623820195401935No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA2395051.268106944876992No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG2284801.2097328855994451No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC2278841.2065772448439425No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG2211641.1709968658557235No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT1979741.0482127901508427No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA1971281.04373347458179No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG1881510.9962029593768437No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1611730.8533625628970561No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA1563210.8276726821156812No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC1530240.8102160586745865No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA1530210.8102001745768238No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA1451740.7686526695291223No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA1385870.7337764855417118No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG1349800.7146785053318151No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA1234100.6534188349607297No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT1215740.6436977671300199No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT1153950.6109818204383227No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT1100500.5826816529246277No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT1044460.5530101583040952No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC1034920.5479590152155891No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT1027450.5440038748727022No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA1022850.5415683132157705No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT1020210.5401705126126619No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA991450.5249429575575849No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT987330.5227615414648548No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC894360.4735367224985644No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC788630.41755586728391564No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT772090.40879843471747007No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA751680.3979919535396494No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT739610.3916012515398309No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA728530.38573472476617815No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG705190.37337689670687707No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC679120.35957361575117963No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC674130.3569315608233342No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC669630.3545489461589445No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA627870.3324382820734085No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT617080.32672530157812746No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG616260.32629113623928313No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG587490.31105828648495193No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG585830.3101793664087548No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT572800.3032803732805332No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT571850.30277737685138423No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC553880.2932628022915882No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT539330.28555901487672825No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG533060.28223923844434534No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT516420.2734288588853578No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG472290.2500633510765765No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT468330.2479666501719136No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG461090.2441332879118733No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA458340.24267724561696855No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG448830.23764198662622504No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA448290.2373560728664983No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT446880.2366095202716562No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG441480.23375038267438858No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG441450.233734498576626No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG413280.21881933077754673No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT394990.2091353258416163No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGG390800.2069168468541068No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC386900.2048519141449691No Hit
CCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTA385770.20425361312924453No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG385760.2042483184299904No Hit
TGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTA380500.20146330662228154No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT375450.1987894834989109No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC373940.19798998391152683No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA370030.19591975650313495No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC357380.18922196194657287No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA357190.1891213626607431No Hit
TACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGA351900.1863204667552717No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA350090.18536212619026163No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG348200.18436142803121797No Hit
AACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC345730.18305363731543076No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA328650.17401029098925844No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT310510.16440570654214098No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA301830.15980990758949604No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG287050.15198434209178954No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG279790.1481403904332409No Hit
TGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA277390.14686966261223305No Hit
CTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT269350.14261272441185685No Hit
CACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAG262880.13918705399438994No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT262380.13892231903167998No Hit
CTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTT250520.13264280571619966No Hit
CACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTA249440.13207097819674615No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT245180.12981543631445727No Hit
TACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACAT243320.1288306222531762No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG240180.12716808668735766No Hit
ACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA239300.12670215315298813No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG228760.1211215401390621No Hit
ACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACA219300.1161127546445896No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGT216310.11452963956758402No Hit
CTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTG216270.11450846077056723No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA207790.11001855580300628No Hit
ATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTC205060.10857310290660986No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG204270.10815482166552813No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA201340.10660347478404775No Hit
AACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTGTA191870.10158939459032106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAC529300.066.048771
TTGTACT1269900.060.9363441
CGCACTA1150.060.86715741
TAGGGAA133150.060.0523451
TGTACTC1313250.059.112432
GCGCTAG800.056.87275339
TGTTACT624100.056.024242
AGGGAAG149950.054.2443242
ACTCTAG1458100.053.153835
GTACTCT1466250.053.0468143
TACTCTA1481500.052.404054
TTTTTCC483200.051.4913671
CTCTAGT1554300.049.9112786
GTTACTA724350.048.270493
TCTAGTT1633700.047.4032337
GAATAAT106800.045.8914571
TTACTAC777600.045.028064
TAATACG110100.044.8544464
CTCGTAT20900.043.3714942
GTATGCC24900.042.58968745
Produced by FastQC (version 0.11.5)