FastQCFastQC Report
Tue 30 Jul 2019
HV2TMBGXB_n01_12_ABF2_R3_RUN1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2TMBGXB_n01_12_ABF2_R3_RUN1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16568853
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT6209913.7479419969505434No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT5746813.4684416597817602No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG4985653.009049570299163No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC4324822.6102108576858036No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA3835512.314891682604704No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG3815382.3027423805377474No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA3733342.2532277883085814No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA3484512.1030484125847457No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA2685201.6206311927566743No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC2377711.435048038630073No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA2278571.3752128768358316No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG2041571.2321734039163725No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG1999171.2065832197316253No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT1881311.1354497502029863No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG1830271.1046449624485173No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA1829861.1043975101957872No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA1653990.9982525646162712No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1543350.9314766689039972No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA1513080.913207450147575No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG1365570.8241789579520079No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA1282740.7741875674797767No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA1277030.7707413422039534No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA1252680.7560450925601186No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT1188950.7175813558126202No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT1150680.6944838004175666No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC1145000.6910556814041382No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT1001200.6042663303247364No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC997520.602045295470966No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT965890.5829552594859765No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT959980.5793883257941874No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA941190.5680477701142017No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT903770.5454632254870027No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA897930.5419385397408016No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA787830.4754885567516351No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC769930.46468515352269707No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC760830.45919292059625366No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT742760.4482869152137447No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT732270.4419557587963392No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT731770.44165398775642467No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC679730.4102456579221265No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT669310.40395674945030896No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA662610.39991301751545505No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC662460.3998224862034807No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC662180.3996534944211286No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG659190.3978489036024401No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG642560.38781199881488476No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG633860.382561182720373No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC602390.3635677134681562No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT582400.3515029072923756No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA574780.3469039166440791No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG546350.32974521531454226No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG515430.3110836942062314No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT503050.30361184325794915No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT489230.29527089171471316No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT478300.2886741767821828No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG469480.28335093563809155No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG459750.27747847120135594No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT436590.26350043663251765No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT422510.2550025641485262No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA422040.25471889937100656No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG417010.251683082709467No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC414900.2504096089210279No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGG414210.24999316488594592No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG408480.24653486876852612No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG401200.24214108242737142No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC397160.2397027724248625No Hit
CCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTA390570.23572543011878977No Hit
TGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTA382130.2306315349650335No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG381510.23025733887553956No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA375030.226346386198248No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG373350.22533243550413537No Hit
AACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC358530.21638794188107044No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA354770.21411862366091367No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT345070.2082642654865729No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT335220.20231937600025782No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA332940.20094330005824787No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT327130.1974367205744417No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG323510.19525189824546094No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC306480.18497357662597405No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG304610.18384495293669392No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA298300.1800366024129733No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA287810.1737054459955677No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA286630.17299326634136955No Hit
CTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT265070.15998089910025756No Hit
AACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTGTA244750.14771692403813347No Hit
TACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGA238530.14396289230159745No Hit
ATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTC235500.14213415979971578No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT231870.1399433020499367No Hit
AGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTA230120.13888710341023605No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT224470.13547709065920255No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCT220900.1333224454342132No Hit
AGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATA219970.1327611512999723No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG219900.13271890335438427No Hit
CTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTCTG217050.13099880842687178No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG216330.13056425812939496No Hit
CTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCA203300.12270010482922383No Hit
TGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA202110.12198188975422741No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA198380.11973067779646544No Hit
CTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTT195380.11792005155697863No Hit
TACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACAT194740.11753378462588812No Hit
TAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGAT188920.11402116972128366No Hit
CCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGT186400.11250024368011473No Hit
CATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACAC181190.10935578944420594No Hit
CACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAG179770.10849875969084885No Hit
ACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACA179280.10820302407173266No Hit
GAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAG175990.10621737062909546No Hit
ACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGAC175500.10592163500997927No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATA175070.10566211191565282No Hit
ACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCT169550.10233055963499707No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTA169500.10230038253100562No Hit
GAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTA168140.10147956530243826No Hit
GTTTGATCAATCTAGGTTACTCTGTAGGGTGTCTATGTACTAGGAGCATA168060.10143128193605194No Hit
CCTACAGAGTAACCTAGATTGATCAAACAGTCCTGATAGTCCTACAGAGG166270.10035094161315813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAC558600.066.711561
TTGTACT1236050.062.7072261
TAGGGAA137200.061.8238031
TGTACTC1272900.061.2052962
TGTTACT645100.057.7478642
AGGGAAG148500.057.6485062
GTACTCT1421050.054.91063
ACTCTAG1441950.053.9888235
GAATAAT99250.053.859821
CGACCTA653.6379788E-1253.8449173
TTTTTCC399000.053.835511
TACTCTA1450900.053.8053474
CTCTAGT1528100.050.8763776
TAGGGCC506.2105755E-848.99769239
GTTACTA762700.048.8345953
TCTAGTT1593450.048.746797
TAATACG114800.045.7614174
TTACTAC816500.045.6728364
ATAATAC121350.043.666083
GCGTATA3150.042.22023411
Produced by FastQC (version 0.11.5)