FastQCFastQC Report
Tue 30 Jul 2019
HV2TMBGXB_n01_10_ABF2_R1_RUN1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2TMBGXB_n01_10_ABF2_R1_RUN1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16505441
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGT5147333.11856556877214No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCT4410962.672427837583982No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGA3741942.267094832546431No Hit
CTGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATG3287651.9918583211439185No Hit
CCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAAC3167691.9191792573127857No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTA3062161.855242765097885No Hit
CCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTA2878701.744091539268778No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGA2580071.563163322930905No Hit
CTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAG2496691.5126466478538805No Hit
CCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTAC2066991.252308253987276No Hit
GTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAG1774901.0753423674047848No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGA1751831.0613651583135524No Hit
TCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAA1681981.0190457801157813No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAG1624730.9843602482357182No Hit
CTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG1552030.9403141667041796No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGA1454440.881188209391073No Hit
GTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGAT1453840.8808246928997535No Hit
TTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAG1452560.8800491910516054No Hit
TAGGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGC1393480.844254933873018No Hit
TTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGA1320610.8001058560022722No Hit
GTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAG1293950.7839536065713119No Hit
CTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTA1158040.7016110626792704No Hit
AAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAA1107470.6709726810692305No Hit
CTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACA1013370.6139611780139652No Hit
GTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCT1000860.6063818591699549No Hit
GGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGT998490.6049459690292431No Hit
ATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCT929870.5633717996386767No Hit
TATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATA902270.5466500410379825No Hit
AGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATT888850.5385193888488045No Hit
GAATAATACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCG867210.5254085607285501No Hit
CTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGAC861930.5222096156049391No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTA814390.49340699227606216No Hit
GTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGAC787200.47693363661110294No Hit
AGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGA784540.4753220468329202No Hit
GGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGT777000.47075385625867255No Hit
GTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTT775870.4700692335333542No Hit
GTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGAT757950.4592122076592804No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTT720080.43626825844883516No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGT689880.4179712617190901No Hit
GGGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCG655540.3971660011992409No Hit
TACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATC643490.3898653783319089No Hit
TACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCC600880.3640496488400401No Hit
TAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACT546350.3310120583872918No Hit
TGTTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGC542030.3283947396497918No Hit
GTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCT536120.3248141022102954No Hit
GTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGAT509270.30854673922375053No Hit
CGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGC492500.29838645329137226No Hit
AACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA459220.2782234052395207No Hit
TGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGT453180.2745640058935717No Hit
CTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTG452590.2742065480104409No Hit
TAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGAT450380.27286759560074764No Hit
CTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAGACACCCTA449790.2725101377176169No Hit
CTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGAC447280.27098942706226387No Hit
ACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAG406210.24610672323144836No Hit
TGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTA404080.24481623968726435No Hit
TAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTG399210.24186569749938824No Hit
GGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT397780.24099931652841025No Hit
TTACTACCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTC388310.23526181457375178No Hit
CTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGG385880.23378957278390808No Hit
AGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTG375620.22757344078234565No Hit
CACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACC372890.22591944074684223No Hit
GAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTG367280.22252056155300545No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAG337680.20458708131457984No Hit
CTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGT324150.19638978443532654No Hit
GTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAG318360.1928818502940939No Hit
GTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGG315500.19114908835213795No Hit
GTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTAT309780.18768356446822598No Hit
ATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAG301330.18256404054881054No Hit
TTTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAG297910.18049199654828976No Hit
CTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCGCCAA297160.18003760093414045No Hit
TGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAA296360.17955291227904788No Hit
CCCTACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTA295170.17883193790459764No Hit
AGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGA290350.17591168875766483No Hit
ACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCA289560.1754330587107609No Hit
CAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAA289070.17513618690951668No Hit
ATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGG286040.17330042862835354No Hit
TGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCA280300.16982278752806423No Hit
GAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTC278670.16883523439331308No Hit
CTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA277540.16815061166799483No Hit
TGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTT268680.16278268481284444No Hit
AACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAAC264340.16015324885896717No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAG258320.15650596672939548No Hit
GTACTCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGG258080.1563605601328677No Hit
GGAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGA257760.15616668467083067No Hit
TACACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACAT255880.15502766633136308No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGA253750.1537371827871791No Hit
CAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAAT253580.15363418644797192No Hit
ACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACA249440.15112592265786778No Hit
TCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGT246460.1493204574176479No Hit
TACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTC238240.1443402814865716No Hit
TCTAGTTGTTACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAG224780.13618539486463888No Hit
GAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATC216100.13092652295688434No Hit
TATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACT203710.12341990741113794No Hit
TGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTG201170.12188102093121898No Hit
CACTCATACTTGGATCACAGCCACTAGTAGCCTATGCTCCTAGTACATAG201000.12177802459201181No Hit
CAGAGGTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGG195030.1181610355033834No Hit
CTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTT185950.11265981926808256No Hit
TACCTCTAATGCTGGACACCTGATAGTCCTACAGAGGTTAGATAGTAGGA184980.1120721342737828No Hit
ATCTAACCTCTGTAGGACTATCAGGACTGTTTGATCAATCTAGGTTACTC178990.1084430279687771No Hit
CGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTGAT178110.10790987044817524No Hit
GTTAGATAGTAGGACTGTTTGATCAATCTAGGTTACTCTGTAGGGTGTCT173090.10486844913746927No Hit
ACGACTCACTATAGGGAGTCTTCGTCGAGTGTTTTCCGCCTCGGCTCTCG171250.10375366523075634No Hit
GAAGAGCATTGTCGAGCAGTTTACCTTCTTCGATTTTGGCGAGAGCCGAG170850.10351132090321005No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTTAC383600.064.620641
TAGGGAA167050.063.8529851
TTGTACT1035750.060.770111
AGGGAAG183200.058.9572942
TGTACTC1076600.058.6637272
TGTTACT457150.054.243572
ACTCTAG1167050.054.062465
GTACTCT1182100.053.4927563
TTTTTCC384300.053.161861
TACTCTA1199600.052.7034844
GAATAAT127150.051.7878271
CTCTAGT1230200.051.2275246
TCTAGTT1294950.048.533177
TAATACG133300.048.4164124
ATAATAC144450.044.9458433
GTTACTA557850.044.4388663
AATACGA154900.041.303485
GGGAAGA260850.041.258883
CTAGTTG1533250.040.8094948
TTACTAC616150.040.285074
Produced by FastQC (version 0.11.5)