FastQCFastQC Report
Tue 30 Jul 2019
HV2MJBGXB_n02_GG037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2MJBGXB_n02_GG037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595056631
Sequences flagged as poor quality0
Sequence length99
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50021080.8406104124230824No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGCATTCAGGCAGCGAGAG18834030.31650819466290425No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA18341400.30822948681669254No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA14322220.2406866717194855No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG13680530.22990299220781224No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA12498260.21003479919207893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT40199000.081.0288541
AGCAGTG43220200.075.120352
GTGGTAT49965350.064.852056
GGTATCA50200950.064.479728
AGTGGTA50566750.064.195525
GCAGTGG50717700.063.981853
GTATCAA50690950.063.775529
CAGTGGT52225300.062.031434
TGGTATC52322900.061.8029827
ATCAACG49586100.035.320110-11
CAACGCA50269250.034.81018412-13
ACGCAGA50630250.034.66015614-15
AGTACAT51138850.034.16707220-21
TACATGG51117600.033.84647422-23
AGAGTAC52139950.033.6822518-19
GCAGAGT53260000.033.00591316-17
TCAACGC49522500.032.63802712-13
TATCAAC49926550.032.46238710-11
CGCAGAG50643800.032.16160616-17
CATGGGG33000950.031.85228224-25
Produced by FastQC (version 0.11.5)