FastQCFastQC Report
Tue 30 Jul 2019
HV2MJBGXB_n01_GG037.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2MJBGXB_n01_GG037.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595056631
Sequences flagged as poor quality0
Sequence length27
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGA2016750.018.463831
GCGCGAT1466600.018.055791
CAATGGA1694050.018.040218
GACTAAC1672250.017.96141
ATGCGAT1887900.017.955941
ACTGATG1625400.017.8729861
GGGAATG1539150.017.8550341
TACGGGC1833800.017.8481921
TGACGGC1490500.017.8191181
CCGGGAT1590800.017.6945721
CTCGAAA1629600.017.6838381
TGGGCGT1429700.017.6799031
CGGACTG1409000.017.6131441
GGGACCT1358350.017.554661
CGTGAGC1306950.017.5410651
TGACTAG1751350.017.5357881
TGAGCCG1551650.017.4390771
CTGCGGA1139700.017.3412631
CGCTGGA1357150.017.2776411
CTCGGGA1556350.017.254821
Produced by FastQC (version 0.11.5)