FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26608598
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4163861.5648550893211284No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG144050.013.5598215
TACACTG106300.013.53968055
CTAGACT70300.012.9801114
TAGACTG84100.012.9226435
TACACCG34850.012.2211155
GTCCTAG51600.011.9509411
CTATACT102550.011.7918124
GTGTAAG79750.011.5044831
GTCTTAC71400.011.4806441
GTGTTAG92200.011.3376181
CTACACT96850.011.2602054
GTATTAG96050.011.1180581
GTATAGA115100.011.1073781
TACTCTG115400.011.0720765
GTCTTAG66100.011.03591251
AGTACTG81600.010.888825
TAGACAG152550.010.7344125
TCTACAC104600.010.5970843
TCTATAC109450.010.5380763
GTCCTAC41500.010.5103661
Produced by FastQC (version 0.11.5)