FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_9361.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_9361.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20298418
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC46250.018.808657145
CCGTATA39850.016.917763145
TATACTG236050.016.2751035
TACACTG170700.015.1170975
GCCGTAA32750.014.830362145
CTATACT147100.014.6358564
TACACCG21500.014.1599845
GTATTAG142550.014.0565931
CTACACT127350.014.002664
GTGTAGG89400.013.8866271
GTCCTAC59300.013.8344521
ACGAACA72450.013.807943145
GTAGGAC60100.013.7533183
GTATAGA142750.013.5791731
GTGTTAG150000.013.552051
TCTATAC145450.013.4594453
GTACTAG62450.013.36914351
CGCCGTT22850.013.324514145
GTGTAAG125200.013.2791211
TACACAG211350.012.9983695
Produced by FastQC (version 0.11.5)