FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_9109.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_9109.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21724681
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG276990.12750014603206372No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG96650.016.9497035
TACACTG128400.016.2585955
TATACTG171900.016.0237165
GGACCGT65200.014.3415356
CTACACT110700.014.3411174
ACGATCC73800.014.144891145
GTATAGG67650.013.8442891
TCTATAC121950.013.7936643
TCTACAC129150.013.7545113
AGGACCG62600.013.5477455
GTATTAG124100.013.5141561
CTATACT119400.013.4783014
GTATAGA124950.013.1898051
CTAGGAC70350.013.0891983
TATACCG37100.012.8951255
GTGTAGG101100.012.8543651
CCTATAC66750.012.8175273
TACACAG153950.012.7598375
CTAGTAC54450.012.6502523
CCGTATA59350.012.580851145
Produced by FastQC (version 0.11.5)