FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_8721.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_8721.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21322667
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259730.12180934026686249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGACA72700.020.34119145
CCGACGC159100.019.865381145
GCCGACC62950.016.927866145
TATACTG237950.015.8404195
CTATACT148750.015.5460274
TACACTG158850.014.3738265
TACACCG36850.014.1626355
GTATTAG151250.014.0642441
CCGTATA46100.013.83765145
GTGTAGG93200.013.2427191
GTATAGG77300.013.1489981
GTCCTAT83500.012.6943521
TCTATAC147800.012.6566743
GTATAGA148950.012.3804771
TACACAG199800.012.3710895
TAGACAG248100.012.2999795
TAGACTG133750.012.2479585
ATACTGT321000.012.2162886
CTAGGAC83250.012.1932113
GTCCTAG84200.011.9852431
Produced by FastQC (version 0.11.5)