FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_8230.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_8230.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20061753
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG294280.14668708163239771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGC155450.020.844995145
GACGACA74950.017.989798145
GCCGACC76650.016.07762145
CCGTATA36400.015.533817145
TACACTG139700.014.5804935
CCTATAC61950.014.2787483
GTGTTAG159550.013.5134221
GTGTAGG102450.013.2505921
TCGGAGA50400.013.2316878
GTAAGAC102550.012.8678893
TCTGTCG96450.012.8514398
CTAGACT103050.012.8030924
CTACACT119000.012.7927474
TATACCG17700.012.6955265
GTATAGG64150.012.674391
TAGACTG122600.012.4753935
GCTCGGA47650.012.474126
GTATTAG108750.012.3494641
GTGTAAG102950.012.3400631
TACAGGT373800.012.2953654
Produced by FastQC (version 0.11.5)