FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_7431.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_7431.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20749754
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG206350.016.0532935
CTATACT157500.015.4649224
TACACTG137100.015.2267575
TATACCG47700.014.7402815
TACACCG60600.014.5928315
GTGTAAG119000.014.3369991
TACACAG167700.013.7018295
GTGTAGG101750.013.5567791
CTACACT115850.013.5159544
TCTATAC158300.013.3287373
TAGACAG196000.013.2767175
CCTACAC101100.013.2677563
TAGACTG111950.013.1439025
CTAGGAC74000.013.1295553
GTGTTAG123050.012.980111
GTATAAG150750.012.81036951
ACACCGT72500.012.7974156
GTATTAG160150.012.5571231
TCTACAC134300.012.47133353
TAGGACT115450.012.4325514
Produced by FastQC (version 0.11.5)