FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_7379.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_7379.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22276469
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG223820.10047373306783942No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG160350.015.27930455
CCGTATA53350.014.267143145
CTACACT125100.013.96528154
TATACTG219250.013.8195065
TACACCG46050.013.6944775
CTTACAC122600.013.5428193
TAGACTG128000.013.4212885
CTATACT154700.013.167584
GTACTAG62400.013.1469131
GTGTAGG100300.013.1010951
GTATAGA159500.013.0632481
GTATTAG151950.012.7089861
TAGACAG251000.012.3602115
GTGTTAG144700.012.3423121
TTACACT183950.012.33486754
TACACAG204350.012.2377135
GTAGGAC67050.012.2192263
TCTATAC152550.012.1197043
GTATAAG146650.012.0296821
GTCTTAG112350.012.0190411
Produced by FastQC (version 0.11.5)