FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_6215.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_6215.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23092727
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC67700.020.559175145
TATACTG270000.016.5379225
CCGTATA49150.016.519148145
CGACGAA65550.015.925128145
CTATACT159950.015.8170994
ACGCGTT31250.015.310418145
GTATAGA160050.015.2413111
TACACTG183800.014.9076885
TCTATAC158050.014.8637793
GACGATA64100.014.249698145
CTACACT134850.013.4929964
GTATAGG80550.013.3393571
GTATTAG151300.013.2916711
CCTACAC98300.013.1294113
TACACCG29400.013.06755
TACACAG218900.012.8484275
TACGCGG43850.012.729586145
TAGACAG271650.012.6483145
ATACTGT366700.012.4140016
CTGCCGC253500.012.410947145