FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_6214.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_6214.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22972154
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG264120.11497398110773592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC36400.020.711796145
CGCCGTT21900.019.860626145
CCGTATA36750.016.569437145
TATACTG239500.016.4040185
CTGCCGC207800.014.442442145
TGACGCG20050.014.10055145
GTGTACG20600.014.0989041
TACGGGT17550.014.0439264
GCCGTAA27400.014.0220375145
TCTATAC154500.013.9854873
CTACACT144700.013.9772714
CGACGAA40500.013.961285145
GGATCGT31050.013.7733728
TACACTG196350.013.7700165
CGGAGAT37600.013.6873839
CTATACT150750.013.4163274
GTATTAG154500.013.3469611
TCGGAGA32400.013.1994838
TACACAG242200.013.0786775
GTACTAG67100.012.9852791
Produced by FastQC (version 0.11.5)