FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_5867.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_5867.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18822034
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG202150.016.351045
CCGACGC135050.015.458944145
TACACTG134950.014.9322825
TACACCG47700.014.43641955
CTATACT130050.014.2692154
CTACACT105850.014.2443594
GTATAGA140700.013.5686271
TCTATAC130550.013.3288783
GTGTAAG111350.013.2988541
GTGTAGG92000.013.0187831
GTCCTAG70950.012.9934831
TACACAG169700.012.9851215
TCTACAC115700.012.9090163
GTATTAG138050.012.8826111
GACGACA67000.012.550619145
CTAGGAC69100.012.2762883
ATACTGT268800.012.1888376
CTTACAC108350.012.1118063
TAGTACT96900.011.9692414
TGTACTG117000.011.9570965
Produced by FastQC (version 0.11.5)