FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_4340.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_4340.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18463380
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG232090.12570287780460565No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT91700.016.0467134
TACACCG89700.015.757125
TACACTG92100.015.5826115
TATACTG148200.015.0149835
GTATAGG93100.014.81518651
CCTACAC91200.014.3893743
CTATACT137750.014.0500874
TATACCG67800.013.7909665
TCTATAC146800.013.7302023
GTGTAGG92550.013.5697831
GTGTAAG88800.013.3253271
TACACAG105550.013.3222685
TATACAG156000.013.2885055
GTCTTAG82250.013.2391031
GTATTAG147550.012.9887681
GTATAAG141200.012.9559441
GTGTTAG82600.012.6556851
TAGACTG72950.012.6186555
CTAGTAC47700.012.615893
GTATAGA150500.012.5894631
Produced by FastQC (version 0.11.5)