FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_3256.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_3256.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23846978
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245310.10286838021991718No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC58600.020.163788145
CCGTATA47200.018.429834145
TATACTG254700.015.9655955
GCTCGGA33900.014.9674776
TCTATAC159700.014.8915963
CGGAGAT46950.014.8211629
ACGAACA98600.014.777511145
TACACTG190750.014.4401015
CGCCGTT36150.014.437977145
ACGAACG34100.014.243035145
TCGGAGA44900.014.04492958
CTATACT167250.014.042834
TACACCG29150.013.9251755
GTGTAGG107600.013.7637021
TACACAG234600.013.5022035
CTGCCGC265900.013.467617145
CTACACT146000.013.30631354
CGGTGGG98800.012.9866905145
GGACCGT21250.012.9620886
TCTCGGG62200.012.819895145
Produced by FastQC (version 0.11.5)