FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30982.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30982.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17557528
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG295530.16832096181193618No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG125850.015.9543595
TATACTG181000.015.2981055
CTACACT100350.013.6533154
CTATACT122200.013.5849474
TATACCG37150.013.4630385
TAGACTG96100.013.3506595
CCTACAC88650.013.3319613
CTAGACT75200.013.1103634
GTGTAGG88200.012.8413511
GTATAGA131400.012.7082891
TCTACAC117650.012.6341813
TACACCG52700.012.516525
GTATTAG132100.012.4760671
TACACAG161300.012.4030235
GTATAGG75250.012.349751
TCTATAC125650.012.2914283
CTTACAC105300.012.1190763
GTCTAGA92850.012.0418581
GCTCGGA43800.011.9154376
GTGTTAG111450.011.7910681
Produced by FastQC (version 0.11.5)