FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30978.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30978.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20662579
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG257910.1248198494486095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC39350.020.079523145
CCGTATA33800.017.58607145
TATACCG18500.016.0641025
TATACTG216150.015.2245665
GACGAAC29850.014.570637145
TACACTG174250.014.4760735
GTGTAAG131100.014.2874061
CCTACAC89850.014.2829273
GTATTAG140700.013.5705681
CGCCGTT23150.013.464465145
CTATACT150500.013.3898834
CTGCCGC149400.013.343007145
CTTACAC124000.013.214443
GTGTAGG88750.013.1702451
GTATACG22050.013.170061
CTACACT132100.013.169734
TCTATAC145950.012.667713
CCTATAC78600.012.6374663
GTGTTAG155900.012.6200051
TACACAG214350.012.6133275
Produced by FastQC (version 0.11.5)