FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30978.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30978.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21145026
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG239690.11335526378638645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA31050.018.677189145
TATACTG222500.016.256655
TACACTG185250.014.986535
CTATACT155500.014.218634
TGACGCG21100.014.085897145
GTATAGG88150.014.0009751
GTGTTAG154100.013.9921841
CGACGAA33850.013.919954145
CTACACT134950.013.8053854
GTGTAGG98300.013.7369751
GTATAGA149750.013.47750951
TACACAG226050.013.4360015
TCTATAC149100.013.3729293
CTGCCGC152900.013.322385145
CTTACAC132850.013.0439113
TCTACAC131550.012.7869983
GTATTAG150000.012.5354581
CGCCGTT21400.012.533463145
CCTATAC80750.012.4808383
CTAGGAC99500.012.387893
Produced by FastQC (version 0.11.5)