FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30976.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30976.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18292291
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG207540.11345763086756054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC26750.018.96958145
GACGAAC21900.016.550432145
TATACTG233300.016.249445
TACACTG165050.014.93189055
TGACGCG15950.014.543628145
CTATACT141700.014.4777554
CTACACT122700.014.47458654
GTGTAGG82250.014.387531
GTCGTAC8201.2551027E-1014.1657861
GTATAGA136600.014.0309711
GTATTAG125450.013.8312611
CCGTATA24650.013.527712145
CCTACAC83150.013.3417373
TCTATAC136450.013.2314983
TACACAG207900.013.179195
CGTACAC16550.013.1433613
GTGTTAG141200.013.059711
ATACTGT302300.012.9960146
CTTACAC113500.012.9683093
CTGCCGC98150.012.703448145
Produced by FastQC (version 0.11.5)