FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30908.TR4_batch_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30908.TR4_batch_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10491834
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG14000.020.7106595
CCGACGC65250.020.553526145
GCCGACC28050.019.124672145
TATACTG127900.017.8526235
GACGACA28250.016.93638145
TACACTG85650.016.5878735
GTACGAC6801.1641532E-1015.9936593
GTATAGA76200.015.2419341
GTCTACG11200.014.9068161
CTAGTAC33450.014.7393483
GTGTAGG48500.014.0689341
GTATACG12900.014.0677721
CCTACAC48550.014.0379643
GTGTAAG64850.013.9918491
CTATACT83000.013.9741914
GTATTAG71200.013.7634981
GTGTTAG75700.013.7124551
CTACACT66650.013.5954824
GTATAGG39550.013.5818931
ATACTGT168350.013.4769356
Produced by FastQC (version 0.11.5)