FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30908.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30908.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18955539
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG219150.016.4060275
TACACTG148250.015.7443365
GTATAGG75700.014.7698071
CTACACT109700.014.4724074
TACACCG35500.014.2933135
CTATACT141750.014.1664164
ACGATCC47100.013.852104145
GTATAGA141100.013.532521
CTAGGAC69950.013.4756773
GTACTAG55950.013.3655581
CCTACAC89000.013.1168893
ATACTGT282750.013.0745686
CTGCCGC184350.012.97672145
TATACCG28100.012.8981345
CTAACAC128250.012.8905793
GTGTAGG88500.012.7976811
TAGACTG117800.012.4299485
TCTATAC139750.012.3486673
GTCCTAG71200.012.33831
GTATTAG130950.012.1973961
Produced by FastQC (version 0.11.5)