FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30904.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30904.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19305130
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205620.1065105492685105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG165650.016.0157685
CTACACT102700.015.1764034
TACACCG77750.014.8236395
CTATACT128600.014.4311124
GTATAGG84100.014.1585421
TATACCG52350.013.985015
GTCCTAG53850.013.8874411
GTATAGA136000.013.8805021
TCTATAC134550.013.8495893
GTGTTAG109850.013.4834421
GTGTAGG94850.013.4724321
GTGTAAG100100.013.3461021
TACACTG115000.013.1736635
CCTACAC97700.012.7650163
TAGACTG97200.012.6031345
GTATTAG146550.012.5839971
TCTACAC128050.012.5707413
CTTACAC96750.012.5156363
GTATAAG130050.012.3382231
CTAACAC107350.012.2929713
Produced by FastQC (version 0.11.5)