FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30903.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30903.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20093249
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG240720.1198014318142377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACACT114900.015.5201864
TATACTG225000.015.2704855
TACACTG148750.014.5703925
CTATACT145600.014.1894434
GTATAGA145500.013.9217451
GTATAGG81700.013.7740651
GTGTTAG135150.013.6448941
GTGTAAG118450.013.4846631
CCTATAC80150.013.2074433
TATACCG30850.013.1579995
TACACAG191150.012.9690695
TATACAG182400.012.9553695
TCTATAC144950.012.8553633
TCTACAC126000.012.429473
GTCCTAC63100.012.4264441
CTTACAC117100.012.3215583
TACACCG47350.012.2469245
CCTACAC99050.012.2245093
GTGTAGG96000.012.1760691
TAGACTG119200.011.6756745
Produced by FastQC (version 0.11.5)