FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30903.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30903.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19627760
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA36250.017.798113145
TATACTG225100.015.7468335
TATACCG18700.015.5052315
TACACTG158700.014.7531745
GCCGACC52250.014.429091145
CTACACT111650.014.154094
GTATTAG138350.014.1150641
TCTATAC136750.013.9442643
CTATACT143150.013.5208534
TACACAG204150.013.3505255
GTCCTAG80000.013.2486811
GTATAGG75100.013.2431221
GTGTAAG122100.013.1397271
CTAGGAC79650.013.0171963
CTTACAC118850.012.8721313
TCTGTCG64250.012.7486468
GTCTAAG83700.012.6630161
TGACGCG27800.012.516658145
GTATAGA140650.012.4390451
GTGTAGG86650.012.3156831
Produced by FastQC (version 0.11.5)