FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30889.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30889.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18391333
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG39850.016.1886545
TATACTG183400.015.4139655
TACACTG136450.015.2991745
GTGTAGG87350.014.5446621
TATACCG35150.014.2289495
CTATACT132300.014.1912184
CTACACT112550.013.9763584
GTATAGG84200.013.1919151
GTATTAG142500.012.8894431
GTATAGA143550.012.8457371
GCCGACC44650.012.826142145
CCTACAC84600.012.5981243
CTGCCGC152550.012.592851145
TCTACAC128700.012.4500943
TAGACAG206250.012.3356565
GTGTAAG115800.012.2878531
TATACAG170150.012.2264125
GTACTAG58100.011.99564551
GTCTAGG73750.011.9110991
GTCTAAG82750.011.8438881
Produced by FastQC (version 0.11.5)