FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30887.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30887.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22239187
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG253100.11380811717622591No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC58950.018.937675145
CGACGAA57750.017.197224145
ACGCGTT29000.016.998112145
TACACTG177950.015.0714975
TATACTG233600.015.0494815
CGGAGAT47750.014.876599
TACGCGG36750.014.794275145
TCGGAGA44500.014.4970868
GTATAGG82700.014.4854091
GTGTAGG101150.014.4272161
CCGTATA46450.014.201867145
CTATACT143250.013.9160234
GACGATA56200.013.801848145
GTATAGA151250.013.536491
CTACACT131900.013.3548354
GTGTAAG132450.013.3200741
GTATTAG149900.013.2709271
GCTCGGA33500.012.9824936
CGGTGGG102900.012.751255145
CTAGGAC94600.012.5697453
Produced by FastQC (version 0.11.5)