FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30852.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30852.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21600567
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG259650.12020517794741221No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA46750.016.281723145
TACACCG31300.015.9792435
TATACTG218700.015.7101745
GTCCTAG85300.014.8104441
TACACTG172800.014.8075235
GCCGACC63550.014.487081145
CTGCCGC222450.014.110687145
GTATAGA149300.014.1028211
GTGTAAG125950.013.7197631
GTGTAGG96050.013.6063921
CGGTGGG90700.013.587336145
CTATACT147400.013.4262854
CGCCGTT41550.013.2597475145
GTATTAG145650.013.1103131
GTGTTAG146400.013.043151
CTACACT128950.012.8737414
CTTACAC126600.012.8295543
GTGTACG32300.012.5879011
TATACAG194850.012.5738345
TACACAG204100.012.5722115
Produced by FastQC (version 0.11.5)