FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_30852.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_30852.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21562145
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG182550.015.2079915
CCGACGC254300.015.108302145
TACACCG84400.014.60028655
TACACTG126200.014.1870645
GTATAGA154800.014.115541
CTACACT108400.013.9763914
GACGACA115800.013.772103145
GTGTAAG112800.013.6435921
TACACAG153050.013.5452675
CTATACT138500.013.4512124
TATACAG178750.013.42260555
GTATTAG148050.013.1900311
TCTATAC152200.013.0527083
GTATAAG155050.012.8754631
TATACCG53750.012.5417865
GTGTTAG116100.012.4429341
TTATACT244000.012.2995324
GTGTAGG106600.012.2579321
TCTACAC141500.012.1950963
CTTACAC109400.012.1945323
Produced by FastQC (version 0.11.5)