FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n02_MG_2397.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n02_MG_2397.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20232727
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG205120.10138030330760653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC49950.020.462828145
CCGTATA35750.018.249395145
TATACTG219150.016.107725
GACGAAC42100.014.119321145
TACACCG26250.014.082755
TACACTG170550.013.812695
GTGTAAG126300.013.4495191
CTGCCGC135750.013.350029145
CTACACT126000.013.2323234
CTATACT142450.013.230914
CTAACAC145000.012.8509793
GACTGCG29450.012.798577
GTATAGG78750.012.7210451
GTGTAGG88550.012.6248561
GTATTAG139850.012.6135721
GTAGCAC77150.012.3113733
CCTACAC91050.012.1837953
TGGACCG23850.012.1567715
TACACAG212050.012.1349135
TATACAG189800.012.1062515
Produced by FastQC (version 0.11.5)