FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26608598
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2540600.9548041576636244No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG80000.020.9823021
TATACTG157000.014.6523035
GTATTAG100950.012.30894851
GTGTAGA88100.012.2072271
TACACTG113600.012.0991175
TACACCG35050.011.76427655
GTGTAAG80600.011.6301631
TAGGACA79700.011.5275474
TAGACAG161150.011.4917995
ATGCCGG129800.011.396592145
CTAGGAC53000.011.3293113
TACACAG154350.011.1075985
GTATAGA110200.011.0779351
CTACACT92600.011.0153844
ACTGTAC123550.011.0057198
ACAGTGC97350.010.921568
TAAGACT122050.010.9061524
CTATACT108650.010.7864064
GTCCTAC41100.010.6081731
TAGACTG87900.010.534155
Produced by FastQC (version 0.11.5)