FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_9361.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_9361.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20298418
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG78450.020.516058145
CTCCGAC35050.020.064003145
CACGAGC86950.016.842854145
CGTCTTG54500.016.628283145
TCTCCGC107750.016.350193145
TATACTG249750.015.9383555
TACACTG168300.015.7678935
CTATACT145950.015.05269054
ATGATCG79250.014.454122145
CGAGACA70550.014.386823145
GTATAGG75600.014.3860241
CTACACT124300.013.7662764
GTGTAGG89550.013.683351
CCTATAC74700.013.588763
CCACGAC48800.013.519339145
TACACAG213050.013.476915
GTATAGA146050.013.2550391
CTAGGAC88750.013.2348513
GTGTAGA128900.013.1623751
GTGTAAG128650.013.0188771
Produced by FastQC (version 0.11.5)