FastQCFastQC Report
Sat 27 Jul 2019
HV2L2BGXB_n01_MG_9109.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV2L2BGXB_n01_MG_9109.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21724681
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG97850.017.116785
GTGTAGG98650.016.7572081
AGACCGC162300.016.751188145
TATACTG166300.016.0881085
TACACTG125300.015.9130165
CCTACAC99000.015.3798463
GTATAGA123550.014.6123651
GTATAGG62300.014.4310371
GAGCCCC191200.014.408826145
ATGCCGG199000.014.354102145
TACACAG145800.014.0236965
CTATACT119500.013.5909864
TCTCCGC192950.013.451512145
TATACAG148800.013.3024195
CTACACT106350.013.2262014
TCTATAC114450.013.0502643
GTATTAG117650.013.0033251
TATACCG38700.012.7399835
TAGACAG175600.012.6760735
GTACTAG57000.012.5928551
Produced by FastQC (version 0.11.5)